Journal: eLife

Article Title: Selenium supplementation inhibits IGF-1 signaling and confers methionine restriction-like healthspan benefits to mice

doi: 10.7554/eLife.62483

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , Free T3 AccuBind ELISA kit , Monobind, Inc , Cat. No:1325–300 , .

Techniques: Enzyme-linked Immunosorbent Assay, Activity Assay, Histone Deacetylase Assay, Recombinant, Software

Journal: Scientific Reports

Article Title: The iodide transporter Slc26a7 impacts thyroid function more strongly than Slc26a4 in mice

doi: 10.1038/s41598-022-15151-4

Figure Lengend Snippet: Serological phenotypes of the deficient mice. ( a ) Serum free thyroxine (FT4), free triiodothyronine (FT3), and thyrotropin (TSH) levels in male wild type (WT), Slc26a7 +/− , and Slc26a7 −/− mice at day 45; n = 4–7 mice of each genotype. One-way analysis of variance (ANOVA) showed significant differences in FT4 and TSH levels (FT4: F (2, 11) = 23.4, p = 0.0001; TSH: F (2, 10) = 22.5, p = 0.0002), but not in FT3 levels ( F (2, 10) = 1.76, p = 0.219). *** p < 0.001 determined by Tukey’s test. ( b ) Serum FT4, FT3, and TSH levels in male WT, Slc26a4 −/− , Slc26a7 −/− , and Slc26a7 del/del Slc26a4 −/− mice at day 90; n = 4–6 mice of each genotype. One-way ANOVA showed significant differences in FT4, FT3, and TSH levels (FT4: F (3, 16) = 22.5, p < 0.0001; FT3: F (3, 14) = 5.29, p = 0.120; TSH: F (3, 14) = 30.3, p < 0.0001). * p < 0.05, *** p < 0.001, **** p < 0.0001 determined by Tukey’s test. FT4 free thyroxine, FT3 free triiodothyronine, TSH thyrotropin, WT wild type.

Article Snippet: Serum free thyroxine (FT4), free triiodothyronine (FT3), and thyrotropin (TSH) concentrations were determined by enzyme-linked immunosorbent assay according to the manufacturer’s instructions (FT4: Mouse Free Thyroxine ELISA Kit CSB-E05080m, CUSABIO, Houston, TX, USA; FT3: Mouse free tri-iodothyronine ELISA kit CSB-E05077m, CUSABIO; TSH: rodent TSH ELISA test kit ERKR7015, Endocrine Technologies, Newark, CA, USA).

Techniques:

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Higher Caloric Exposure in Critically Ill Patients Transiently Accelerates Thyroid Hormone Activation

doi: 10.1210/clinem/dgz077

Figure Lengend Snippet: Baseline Values for Outcome Variables

Article Snippet: Blood was collected in 2 EDTA tubes, centrifuged within 10 minutes of collection and frozen to −70° C. The plasma samples were later thawed and TSH, total T3, and total T4 were quantified through immunoassay kits purchased from Monobind Inc. in Lake Forest, CA.

Techniques:

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Higher Caloric Exposure in Critically Ill Patients Transiently Accelerates Thyroid Hormone Activation

doi: 10.1210/clinem/dgz077

Figure Lengend Snippet: Intent-to-Treat Model Exploring Effect of Being in the 100% ECN vs 40% ECN Group on T3/rT3 Adjusting for Baseline T3/rT3 Ratio Over Days 1 to 7

Article Snippet: Blood was collected in 2 EDTA tubes, centrifuged within 10 minutes of collection and frozen to −70° C. The plasma samples were later thawed and TSH, total T3, and total T4 were quantified through immunoassay kits purchased from Monobind Inc. in Lake Forest, CA.

Techniques:

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Higher Caloric Exposure in Critically Ill Patients Transiently Accelerates Thyroid Hormone Activation

doi: 10.1210/clinem/dgz077

Figure Lengend Snippet: Fitted curves for the effect of feeding group on plasma T3/rT3 ratio (3a), plasma T3 (3b), plasma rT3(3c), and plasma TSH (3d).

Article Snippet: Blood was collected in 2 EDTA tubes, centrifuged within 10 minutes of collection and frozen to −70° C. The plasma samples were later thawed and TSH, total T3, and total T4 were quantified through immunoassay kits purchased from Monobind Inc. in Lake Forest, CA.

Techniques:

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Higher Caloric Exposure in Critically Ill Patients Transiently Accelerates Thyroid Hormone Activation

doi: 10.1210/clinem/dgz077

Figure Lengend Snippet: Intent-to-Treat Model Exploring Effect of Being in the 100% ECN vs 40% ECN Group on Total T3 Over Days 1 to 7

Article Snippet: Blood was collected in 2 EDTA tubes, centrifuged within 10 minutes of collection and frozen to −70° C. The plasma samples were later thawed and TSH, total T3, and total T4 were quantified through immunoassay kits purchased from Monobind Inc. in Lake Forest, CA.

Techniques:

Journal: The Journal of Clinical Endocrinology and Metabolism

Article Title: Higher Caloric Exposure in Critically Ill Patients Transiently Accelerates Thyroid Hormone Activation

doi: 10.1210/clinem/dgz077

Figure Lengend Snippet: Intent-to-Treat Model Exploring Effect of Being in the 100% ECN vs 40% ECN Group on Reverse T3 Over Days 1 to 7

Article Snippet: Blood was collected in 2 EDTA tubes, centrifuged within 10 minutes of collection and frozen to −70° C. The plasma samples were later thawed and TSH, total T3, and total T4 were quantified through immunoassay kits purchased from Monobind Inc. in Lake Forest, CA.

Techniques: